BioTechniques is a MEDLINE-indexed open access journal providing the life science research community with aninvaluable resource for accessing the latest methods, techniques and protocols. Alongside peer-reviewed methods-based research and review articles, the journal publishes news and opinion articles covering experimental design, interesting observations and the latest tools available to the laboratory researcher.

At BioTechniques, we encourage all authors to submit a detailed protocol alongside any new method or technique published in the journal. Our opinion has always been that having a single document that carefully lists the necessary reagents, materials, key steps, hints and troubleshooting tips for each protocol is vital for its successful application in the lab.

It is important to ensure that these protocols are easily reusable and reproducible, providing users with detailed instructions for performing their experiments.

In this edition of the Protocol Guide, we have collated the top protocols from methods published in BioTechniques throughout 2020 on cell culture, cell biology and microscopy.