Technical Note highlights:
- It describes how the CellDrop Automated Cell Counter uses dual fluorescence to distinguish between isolated nuclei, unlysed cells and debris to provide accurate counts for single nuclear RNA-Sequencing or ATAC-Seq workflows
- The method will label successfully isolated nuclei red and any remaining intact cells green. This allows the user to calculate the residual intact cells that carryover as a percent of total counted and determine if the experimental workflow can proceed.
- Compares nuclei counting data using dual fluorescence with traditional trypan blue staining demonstrating significant improvements in differentiation of nuclei, cells and debris to improve library preparation.
